This article offers insights into gender relations at every node of the maize value chain in rural Mozambique. Data were collected using mixed methods, including a survey of 295 households, key informant interviews with 29 individuals (breeders, agro-dealers, traders, and processors), and 12 sex-disaggregated focus group discussions with smallholder farmers. The findings show that in terms of the gender division of labor in maize production, there is no significant difference between male-headed and female-headed households concerning the partici- pation of men, women, children, and hired labor. In addition, due to their culturally prescribed role as head of household, men are responsible for maize marketing and for making decisions both at the farm level and across the higher nodes of the value chain. Moreover, cultural restrictions on women’s mobility and gender disparities in access to transportation tend to exclude women from participating in the markets. However, women from matrilineal villages are shown to have more autonomy than those from patrilineal villages in making decisions about the quantity of maize to sell, participation in the market, and control of revenue. Finally, whether the woman belongs to the patriarchal or matriarchal system, shestill faceschallengesasshe accedestothe higher nodes of the value chain, for example, participating as a trader, because of her dual roles as mother/homemaker and business woman.